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ARID5A Antikörper (C-Term)

ARID5A Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Kaninchen, Affe WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6741610
  • Target Alle ARID5A Antikörper anzeigen
    ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))
    Bindungsspezifität
    • 7
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    C-Term
    Reaktivität
    • 27
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Hund, Meerschweinchen, Kaninchen, Affe
    Wirt
    • 22
    • 5
    Kaninchen
    Klonalität
    • 24
    • 3
    Polyklonal
    Konjugat
    • 17
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser ARID5A Antikörper ist unkonjugiert
    Applikation
    • 26
    • 15
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human ARID5A
    Homologie
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit (100%) Pig (93%) Horse (91%) Guinea pig (85%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic peptide from C-Terminus of human ARID5A (Q03989, NP_997646). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Elephant, Dog, Rabbit (100%), Bat, Pig (93%), Panda, Horse (91%), Marmoset, Guinea pig (85%), Bovine (80%).

    Type of Immunogen: Synthetic peptide
    Isotyp
    IgG
  • Applikationshinweise
    Approved: IHC, IHC-P (5 μg/mL), WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Distilled Water.
    Konzentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handhabung
    Avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))
    Andere Bezeichnung
    MRF-1 / ARID5A (ARID5A Produkte)
    Synonyme
    MRF-1 antikoerper, MRF1 antikoerper, RP11-363D14 antikoerper, D430024K22Rik antikoerper, Mrf1 antikoerper, AT-rich interaction domain 5A antikoerper, AT rich interactive domain 5A (MRF1-like) antikoerper, ARID5A antikoerper, Arid5a antikoerper, arid5a antikoerper
    Hintergrund
    Name/Gene ID: ARID5A

    Synonyms: ARID5A, Modulator recognition factor 1, MRF-1, MRF1, RFVG5814, RP11-363D14, Modulator recognition factor I
    Gen-ID
    10865
    NCBI Accession
    NP_997646
    UniProt
    Q03989
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