ARID5A Antikörper (C-Term)
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- Target Alle ARID5A Antikörper anzeigen
- ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))
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Bindungsspezifität
- C-Term
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Reaktivität
- Human, Maus, Ratte, Hund, Kaninchen, Meerschweinchen, Affe
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser ARID5A Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human ARID5A
- Homologie
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit (100%) Pig (93%) Horse (91%) Guinea pig (85%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic peptide from C-Terminus of human ARID5A (Q03989, NP_997646). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Elephant, Dog, Rabbit (100%), Bat, Pig (93%), Panda, Horse (91%), Marmoset, Guinea pig (85%), Bovine (80%).
Type of Immunogen: Synthetic peptide - Isotyp
- IgG
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- Applikationshinweise
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Distilled Water.
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- ARID5A (AT Rich Interactive Domain 5A (MRF1-Like) (ARID5A))
- Andere Bezeichnung
- MRF-1 / ARID5A (ARID5A Produkte)
- Synonyme
- MRF-1 antikoerper, MRF1 antikoerper, RP11-363D14 antikoerper, D430024K22Rik antikoerper, Mrf1 antikoerper, AT-rich interaction domain 5A antikoerper, AT rich interactive domain 5A (MRF1-like) antikoerper, ARID5A antikoerper, Arid5a antikoerper, arid5a antikoerper
- Hintergrund
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Name/Gene ID: ARID5A
Synonyms: ARID5A, Modulator recognition factor 1, MRF-1, MRF1, RFVG5814, RP11-363D14, Modulator recognition factor I - Gen-ID
- 10865
- NCBI Accession
- NP_997646
- UniProt
- Q03989
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