BMPER Antikörper (AA 501-550)

Produktnummer ABIN6741613

Produktdetails anti-BMPER Antikörper

Target: BMPER (BMP Binding Endothelial Regulator (BMPER))

Bindungsspezifität: AA 501-550

Reaktivität: Human, Maus, Ratte, Schwein, Kaninchen, Hund, Zebrafisch (Danio rerio), Pferd, Fledermaus, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BMPER Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human BMPER

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Chicken (100%) Bovine (92%) Xenopus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa501-550 of human BMPER (Q8N8U9, NP_597725). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bat, Rabbit, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Lizard (100%), Marmoset, Bovine, Guinea pig (92%), Xenopus, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu BMPER

Target: BMPER (BMP Binding Endothelial Regulator (BMPER))

Andere Bezeichnung: BMPER (BMPER Produkte)

Hintergrund: Name/Gene ID: BMPER

Synonyms: BMPER, CRIM3, Crossveinless 2, CV-2, Crossveinless-2, HCV2, Protein crossveinless-2, CV2, KIAA1965

Gen-ID: 168667

NCBI Accession: NP_597725

UniProt: Q8N8U9