Ubiquilin 1 Antikörper (AA 290-339)

Produktnummer ABIN6741620

Produktdetails anti-Ubiquilin 1 Antikörper

Target: Ubiquilin 1 (UBQLN1)

Bindungsspezifität: AA 290-339

Reaktivität: Human, Pferd, Kaninchen, Schaf, Rind (Kuh), Hund, Meerschweinchen, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ubiquilin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human UBQLN1

Homologie: Percent identity by BLAST analysis: Human, Sheep, Dog, Bovine, Rabbit, Horse (100%) Mouse, Rat, Chicken (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa290-339 of human UBQLN1 (Q5T6J9, NP_038466). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Sheep, Elephant, Dog, Bovine, Rabbit, Horse, Guinea pig, Platypus (100%), Mouse, Rat, Panda, Bat (92%), Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Ubiquilin 1

Target: Ubiquilin 1 (UBQLN1)

Andere Bezeichnung: UBQLN1 / Ubiquilin (UBQLN1 Produkte)

Synonyme: UBQLN1 antikoerper, DA41 antikoerper, DSK2 antikoerper, PLIC-1 antikoerper, UBQN antikoerper, XDRP1 antikoerper, 1110046H03Rik antikoerper, 1810030E05Rik antikoerper, AU019746 antikoerper, C77538 antikoerper, D13Ertd372e antikoerper, Da41 antikoerper, Dsk2 antikoerper, Plic-1 antikoerper, Plic1 antikoerper, Xdrp1 antikoerper, ubiquilin 1 antikoerper, UBQLN1 antikoerper, Ubqln1 antikoerper

Hintergrund: Name/Gene ID: UBQLN1

Synonyms: UBQLN1, DA41, HPLIC-1, PLIC-1, Ubiquilin, UBQN, XDRP1, DSK2, PLIC1, Ubiquilin 1, Ubiquilin-1

Gen-ID: 29979

NCBI Accession: NP_038466

UniProt: Q9UMX0