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ZNF777 Antikörper (AA 468-517)

ZNF777 Reaktivität: Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Schwein WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6741624
  • Target Alle ZNF777 Antikörper anzeigen
    ZNF777 (Zinc Finger Protein 777 (ZNF777))
    Bindungsspezifität
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 468-517
    Reaktivität
    • 10
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Human, Maus, Ratte, Rind (Kuh), Meerschweinchen, Pferd, Kaninchen, Schwein
    Wirt
    • 8
    • 2
    Kaninchen
    Klonalität
    • 10
    Polyklonal
    Konjugat
    • 10
    Dieser ZNF777 Antikörper ist unkonjugiert
    Applikation
    • 10
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human ZNF777
    Homologie
    Percent identity by BLAST analysis: Human, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog (92%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa468-517 of human ZNF777 (Q9ULD5, NP_056509). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Gorilla, Marmoset, Dog, Bat (92%).

    Type of Immunogen: Synthetic peptide
    Isotyp
    IgG
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  • Applikationshinweise
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Distilled water
    Konzentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handhabung
    Avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    ZNF777 (Zinc Finger Protein 777 (ZNF777))
    Andere Bezeichnung
    ZNF777 (ZNF777 Produkte)
    Synonyme
    2500002G23Rik antikoerper, ZNF777 antikoerper, RGD1566056 antikoerper, Znf777 antikoerper, zinc finger protein 777 antikoerper, Zfp777 antikoerper, ZNF777 antikoerper, LOC769049 antikoerper
    Hintergrund
    Name/Gene ID: ZNF777
    Family: Zinc Finger

    Synonyms: ZNF777, Zinc finger protein 777, KIAA1285
    Gen-ID
    27153
    NCBI Accession
    NP_056509
    UniProt
    Q9ULD5
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