MKNK2 Antikörper (AA 36-85)

Produktnummer ABIN6744440

Produktdetails anti-MKNK2 Antikörper

Target: MKNK2 (MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2))

Bindungsspezifität: AA 36-85

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Pferd, Zebrafisch (Danio rerio), Fledermaus, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MKNK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Marke: IHC-plus™

Spezifität: Human MKNK2 / MNK2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Pig, Guinea pig, Zebrafish (100%) Bovine (92%) Xenopus (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human MKNK2 (Q9HBH9, NP_951009). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Pig, Opossum, Guinea pig, Zebra finch, Stickleback, Zebrafish (100%), Bovine (92%), Xenopus (91%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu MKNK2

Target: MKNK2 (MAP Kinase Interacting serine/threonine Kinase 2 (MKNK2))

Andere Bezeichnung: MKNK2 / MNK2 (MKNK2 Produkte)

Synonyme: GPRK7 antikoerper, MNK2 antikoerper, gprk7 antikoerper, mnk2 antikoerper, 2010016G11Rik antikoerper, Gprk7 antikoerper, Mnk2 antikoerper, mknk2 antikoerper, wu:fb37e05 antikoerper, wz5090 antikoerper, fi34c11 antikoerper, wu:fi34c11 antikoerper, wu:fi41e12 antikoerper, zgc:55587 antikoerper, MAP kinase interacting serine/threonine kinase 2 antikoerper, MAP kinase-interacting serine/threonine kinase 2 antikoerper, MAP kinase interacting serine/threonine kinase 2b antikoerper, MAP kinase interacting serine/threonine kinase 2 L homeolog antikoerper, MAP kinase interacting serine/threonine kinase 2a antikoerper, MKNK2 antikoerper, mknk2 antikoerper, Mknk2 antikoerper, mknk2b antikoerper, mknk2.L antikoerper, mknk2a antikoerper

Hintergrund: Name/Gene ID: MKNK2
Subfamily: MNK
Family: Protein Kinase

Synonyms: MKNK2, GPRK7, MNK2, MAPK-Interacting Kinase 2

Gen-ID: 2872

NCBI Accession: NP_951009

UniProt: Q9HBH9

Pathways: MAPK Signalweg