Tyrosinase-Related Protein 1 Antikörper (AA 396-445)

Produktnummer ABIN490060

Produktdetails anti-Tyrosinase-Related Protein 1 Antikörper

Target: Tyrosinase-Related Protein 1 (TYRP1)

Bindungsspezifität: AA 396-445

Reaktivität: Human, Maus, Ratte, Hund, Ziege, Pferd, Kaninchen, Schaf, Meerschweinchen, Zebrafisch (Danio rerio), Affe, Huhn, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Tyrosinase-Related Protein 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human TYRP1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Sheep, Dog, Goat, Rabbit, Horse, Guinea pig (100%) Bovine, Pig, Chicken (92%) Zebrafish (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa396-445 of human TYRP1 (P17643, NP_000541). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Goat, Panda, Dog, Cat, Bat, Rabbit, Horse, Guinea pig, Chicken, Platypus (100%), Elephant, Bovine, Pig, Opossum, Zebra finch, Lizard (92%), Xenopus (85%), Pufferfish, Zebrafish (83%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Tyrosinase-Related Protein 1

Target: Tyrosinase-Related Protein 1 (TYRP1)

Andere Bezeichnung: TYRP1 / gp75 (TYRP1 Produkte)

Hintergrund: Name/Gene ID: TYRP1

Synonyms: TYRP1, B-PROTEIN, CAS2, CATB, Glycoprotein 75, Hstyrrp, gp75, OCA3, SHEP11, TRP-1, Tyrosinase-related protein 1, TYRP, TYRRP, Catalase B, DHICA oxidase, Melanoma antigen gp75

Gen-ID: 7306

NCBI Accession: NP_000541

UniProt: P17643