TRPM2 Antikörper (AA 431-480)
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- Target Alle TRPM2 Antikörper anzeigen
- TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))
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Bindungsspezifität
- AA 431-480
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Reaktivität
- Human, Maus, Ratte, Meerschweinchen
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser TRPM2 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human TRPM2
- Homologie
- Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Bovine, Horse (92%) Guinea pig (87%) Chicken (84%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa431-480 of human TRPM2 (O94759, NP_003298). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat (100%), Galago, Marmoset, Elephant, Bovine, Bat, Horse (92%), Guinea pig (87%), Panda, Platypus, Lizard (85%), Turkey, Zebra finch, Chicken (84%).
Type of Immunogen: Synthetic peptide - Isotyp
- IgG
- Top Product
- Discover our top product TRPM2 Primärantikörper
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- Applikationshinweise
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Approved: IHC, IHC-P (10 - 20 μg/mL), WB (1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Distilled water
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))
- Andere Bezeichnung
- TRPM2 (TRPM2 Produkte)
- Synonyme
- EREG1 antikoerper, KNP3 antikoerper, LTRPC2 antikoerper, NUDT9H antikoerper, NUDT9L1 antikoerper, TRPC7 antikoerper, Trpm2-predicted antikoerper, TRPM2 antikoerper, si:ch73-263b18.1 antikoerper, 9830168K16Rik antikoerper, C79133 antikoerper, Trp7 antikoerper, Trrp7 antikoerper, transient receptor potential cation channel subfamily M member 2 antikoerper, transient receptor potential cation channel, subfamily M, member 2 antikoerper, transient receptor potential cation channel subfamily C member 7 antikoerper, TRPM2 antikoerper, Trpm2 antikoerper, trpm2 antikoerper, TRPC7 antikoerper
- Hintergrund
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Name/Gene ID: TRPM2
Subfamily: Calcium channel - TRP
Family: Ion Channel
Synonyms: TRPM2, EREG1, LTrpC-2, LTRPC2, NUDT9L1, KNP3, NUDT9H, Trrp7 - Gen-ID
- 7226
- NCBI Accession
- NP_003298
- UniProt
- O94759
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