LIMD1 Antikörper (N-Term)

Produktnummer ABIN6744453

Produktdetails anti-LIMD1 Antikörper

Target: LIMD1 (LIM Domains Containing 1 (LIMD1))

Bindungsspezifität: N-Term

Reaktivität: Maus, Human, Ratte, Rind (Kuh), Meerschweinchen, Schwein, Kaninchen, Zebrafisch (Danio rerio), Affe, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIMD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human LIMD1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Pig (100%) Dog (92%) Rabbit (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human LIMD1 (Q9UGP4). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Bovine, Pig, Guinea pig, Platypus, Zebrafish (100%), Elephant, Panda, Dog, Bat, Opossum (92%), Rabbit, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu LIMD1

Target: LIMD1 (LIM Domains Containing 1 (LIMD1))

Andere Bezeichnung: LIMD1 (LIMD1 Produkte)

Synonyme: AW822033 antikoerper, D9Ertd192e antikoerper, xlimd1 antikoerper, LIM domains containing 1 antikoerper, LIM domains containing 1 L homeolog antikoerper, LIMD1 antikoerper, Limd1 antikoerper, limd1 antikoerper, limd1.L antikoerper

Hintergrund: Name/Gene ID: LIMD1

Synonyms: LIMD1, LIM domains containing 1

Gen-ID: 8994

UniProt: Q9UGP4

Pathways: Ribonucleoprotein Complex Subunit Organization