FUBP3 Antikörper (AA 503-552)

Produktnummer ABIN6744454

Produktdetails anti-FUBP3 Antikörper

Target: FUBP3 (Far Upstream Element (FUSE) Binding Protein 3 (FUBP3))

Bindungsspezifität: AA 503-552

Reaktivität: Human, Maus, Ratte, Meerschweinchen, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FUBP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human FUBP3 / FBP3

Homologie: Percent identity by BLAST analysis: Human (100%) Bovine, Horse, Pig (92%) Mouse, Rat, Dog (85%) Guinea pig (84%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa503-552 of human FUBP3 (Q96I24, NP_003925). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%), Monkey, Galago, Marmoset, Elephant, Bovine, Bat, Horse, Pig (92%), Mouse, Rat, Dog (85%), Guinea pig (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu FUBP3

Target: FUBP3 (Far Upstream Element (FUSE) Binding Protein 3 (FUBP3))

Andere Bezeichnung: FUBP3 (FUBP3 Produkte)

Synonyme: FBP3 antikoerper, Marta2 antikoerper, A330051M14Rik antikoerper, AV006371 antikoerper, zgc:101598 antikoerper, far upstream element binding protein 3 antikoerper, far upstream element-binding protein 3 antikoerper, far upstream element binding protein 3 S homeolog antikoerper, far upstream element (FUSE) binding protein 3 antikoerper, FUBP3 antikoerper, LOC100196681 antikoerper, Fubp3 antikoerper, fubp3.S antikoerper, fubp3 antikoerper

Hintergrund: Name/Gene ID: FUBP3

Synonyms: FUBP3, FBP3, FUSE-binding protein 3

Gen-ID: 8939

NCBI Accession: NP_003925

UniProt: Q96I24