EHD4 Antikörper (AA 396-445)

Produktnummer ABIN6744457

Produktdetails anti-EHD4 Antikörper

Target: EHD4 (EH-Domain Containing 4 (EHD4))

Bindungsspezifität: AA 396-445

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Rind (Kuh), Meerschweinchen, Kaninchen, Fledermaus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EHD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human EHD4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa396-445 of human EHD4 (Q9H223, NP_644670). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Panda, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Platypus, Lizard (100%), Elephant, Turkey, Zebra finch (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu EHD4

Target: EHD4 (EH-Domain Containing 4 (EHD4))

Andere Bezeichnung: EHD4 (EHD4 Produkte)

Synonyme: EHD4 antikoerper, past4 antikoerper, PAST4 antikoerper, 2210022F10Rik antikoerper, AI197390 antikoerper, AI846352 antikoerper, AV006278 antikoerper, Past2 antikoerper, EH domain containing 4 antikoerper, EH-domain containing 4 antikoerper, EH domain containing 4 L homeolog antikoerper, EHD4 antikoerper, ehd4 antikoerper, ehd4.L antikoerper, Ehd4 antikoerper

Hintergrund: Name/Gene ID: EHD4

Synonyms: EHD4, EH domain-containing protein 4, EH-domain containing 4, EH domain containing 4, HCA10, HCA11, PAST4, Ortholog of rat pincher, PAST homolog 4

Gen-ID: 30844

NCBI Accession: NP_644670

UniProt: Q9H223