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EHD4 Antikörper (AA 396-445)

EHD4 Reaktivität: Human, Maus, Ratte, Hund, Pferd, Rind (Kuh), Meerschweinchen, Kaninchen, Fledermaus, Affe WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6744457
  • Target Alle EHD4 Antikörper anzeigen
    EHD4 (EH-Domain Containing 4 (EHD4))
    Bindungsspezifität
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 396-445
    Reaktivität
    • 16
    • 8
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Hund, Pferd, Rind (Kuh), Meerschweinchen, Kaninchen, Fledermaus, Affe
    Wirt
    • 14
    • 2
    Kaninchen
    Klonalität
    • 14
    • 2
    Polyklonal
    Konjugat
    • 14
    • 1
    • 1
    Dieser EHD4 Antikörper ist unkonjugiert
    Applikation
    • 16
    • 7
    • 4
    • 3
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human EHD4
    Homologie
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig (100%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa396-445 of human EHD4 (Q9H223, NP_644670). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Panda, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Platypus, Lizard (100%), Elephant, Turkey, Zebra finch (92%).

    Type of Immunogen: Synthetic peptide
    Isotyp
    IgG
    Top Product
    Discover our top product EHD4 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Distilled water
    Konzentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handhabung
    Avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    EHD4 (EH-Domain Containing 4 (EHD4))
    Andere Bezeichnung
    EHD4 (EHD4 Produkte)
    Synonyme
    EHD4 antikoerper, past4 antikoerper, PAST4 antikoerper, 2210022F10Rik antikoerper, AI197390 antikoerper, AI846352 antikoerper, AV006278 antikoerper, Past2 antikoerper, EH domain containing 4 antikoerper, EH-domain containing 4 antikoerper, EH domain containing 4 L homeolog antikoerper, EHD4 antikoerper, ehd4 antikoerper, ehd4.L antikoerper, Ehd4 antikoerper
    Hintergrund
    Name/Gene ID: EHD4

    Synonyms: EHD4, EH domain-containing protein 4, EH-domain containing 4, EH domain containing 4, HCA10, HCA11, PAST4, Ortholog of rat pincher, PAST homolog 4
    Gen-ID
    30844
    NCBI Accession
    NP_644670
    UniProt
    Q9H223
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