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SH3BP2 Antikörper (AA 432-481)

SH3BP2 Reaktivität: Human, Maus, Ratte, Affe, Rind (Kuh) WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6744458
  • Target Alle SH3BP2 Antikörper anzeigen
    SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))
    Bindungsspezifität
    • 7
    • 7
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 432-481
    Reaktivität
    • 39
    • 12
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Affe, Rind (Kuh)
    Wirt
    • 35
    • 4
    • 1
    Kaninchen
    Klonalität
    • 38
    • 2
    Polyklonal
    Konjugat
    • 21
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser SH3BP2 Antikörper ist unkonjugiert
    Applikation
    • 27
    • 20
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human SH3BP2
    Homologie
    Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Rabbit, Guinea pig (92%) Bovine (84%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa432-481 of human SH3BP2 (P78314, NP_003014). Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Mouse, Rat, Opossum (100%), Elephant, Bat, Rabbit, Guinea pig (92%), Galago (85%), Bovine (84%), Zebra finch (83%).

    Type of Immunogen: Synthetic peptide
    Isotyp
    IgG
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  • Applikationshinweise
    Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Distilled water
    Konzentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handhabung
    Avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    SH3BP2 (SH3-Domain Binding Protein 2 (SH3BP2))
    Andere Bezeichnung
    SH3BP2 (SH3BP2 Produkte)
    Synonyme
    SH3BP2 antikoerper, 3BP-2 antikoerper, 3BP2 antikoerper, CRBM antikoerper, CRPM antikoerper, SH3 domain binding protein 2 antikoerper, SH3-domain binding protein 2 antikoerper, SH3BP2 antikoerper, Sh3bp2 antikoerper
    Hintergrund
    Name/Gene ID: SH3BP2

    Synonyms: SH3BP2, Abl-SH3 binding protein 2, 3BP-2, 3BP2, Cherubism, CRPM, RES4-23, SH3 domain-binding protein 2, TNFAIP3 interacting protein 2, CRBM, SH3-domain binding protein 2
    Gen-ID
    6452
    NCBI Accession
    NP_003014
    UniProt
    P78314
    Pathways
    T-Zell Rezeptor Signalweg
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