PGLS Antikörper (C-Term)

Produktnummer ABIN6744461

Produktdetails anti-PGLS Antikörper

Target: PGLS (6-phosphogluconolactonase (PGLS))

Bindungsspezifität: C-Term

Reaktivität: Human, Schwein, Rind (Kuh), Hund, Pferd, Fledermaus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PGLS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human PGLS

Homologie: Percent identity by BLAST analysis: Dog (100%) Mouse, Rat, Guinea pig (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human PGLS (O95336, NP_036220). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Marmoset, Elephant, Dog, Bovine, Bat, Horse, Pig (100%), Gibbon, Mouse, Rat, Panda, Guinea pig (92%), Chicken, Platypus (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu PGLS

Target: PGLS (6-phosphogluconolactonase (PGLS))

Andere Bezeichnung: PGLS / 6PGL (PGLS Produkte)

Synonyme: 6PGL antikoerper, PGLS antikoerper, PSPTO1301 antikoerper, 1110030K05Rik antikoerper, AI447866 antikoerper, Plgs antikoerper, 6-phosphogluconolactonase antikoerper, PGLS antikoerper, pgl antikoerper, CND03390 antikoerper, CNE04030 antikoerper, Tb11.02.4200 antikoerper, Pgls antikoerper

Hintergrund: Name/Gene ID: PGLS

Synonyms: PGLS, 6PGL, 6-phosphogluconolactonase

Gen-ID: 25796

NCBI Accession: NP_036220

UniProt: O95336