FOXM1 Antikörper (C-Term)

Produktnummer ABIN490076

Produktdetails anti-FOXM1 Antikörper

Target: FOXM1 (Forkhead Box M1 (FOXM1))

Bindungsspezifität: C-Term

Reaktivität: Human, Hund, Meerschweinchen, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FOXM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human FOXM1

Homologie: Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Rabbit (91%) Dog, Pig, Guinea pig (83%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human FOXM1 (Q08050, NP_068772). Percent identity by BLAST analysis: Human, Chimpanzee (100%), Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Rabbit (91%), Elephant (90%), Galago, Panda, Dog, Pig, Guinea pig (83%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu FOXM1

Target: FOXM1 (Forkhead Box M1 (FOXM1))

Andere Bezeichnung: FOXM1 (FOXM1 Produkte)

Synonyme: FKHL16 antikoerper, FOXM1B antikoerper, HFH-11 antikoerper, HFH11 antikoerper, HNF-3 antikoerper, INS-1 antikoerper, MPHOSPH2 antikoerper, MPP-2 antikoerper, MPP2 antikoerper, PIG29 antikoerper, TGT3 antikoerper, TRIDENT antikoerper, FOXM1 antikoerper, AA408308 antikoerper, AW554517 antikoerper, BB238854 antikoerper, Fkh16 antikoerper, Foxm1b antikoerper, HFH-11B antikoerper, Mpm2 antikoerper, WIN antikoerper, Trident antikoerper, fkhl16 antikoerper, foxm1l antikoerper, hfh-11 antikoerper, mpp2 antikoerper, zgc:63854 antikoerper, forkhead box M1 antikoerper, forkhead box M1 S homeolog antikoerper, FOXM1 antikoerper, Foxm1 antikoerper, foxm1 antikoerper, foxm1.S antikoerper

Hintergrund: Name/Gene ID: FOXM1
Family: Transcription factor

Synonyms: FOXM1, Forkhead box M1, Forkhead box protein M1, HFH11, HNF-3, HNF-3/fork-head homolog 11, INS-1, FOXM1B, M-phase phosphoprotein 2, MPP-2, MPHOSPH2, PIG29, TRIDENT, FKHL16, HFH-11, TGT3, Transcription factor Trident, WIN

Gen-ID: 2305

NCBI Accession: NP_068772

UniProt: Q08050

Pathways: Positive Regulation of Response to DNA Damage Stimulus