PDE8A Antikörper (AA 551-600)

Produktnummer ABIN6746531

Produktdetails anti-PDE8A Antikörper

Target: PDE8A (phosphodiesterase 8A (PDE8A))

Bindungsspezifität: AA 551-600

Reaktivität: Human, Ratte, Affe, Kaninchen, Hund, Pferd, Rind (Kuh), Meerschweinchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDE8A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: Human PDE8A

Homologie: Percent identity by BLAST analysis: Human, Dog, Bovine, Rabbit, Horse, Guinea pig (100%) Mouse, Rat, Chicken, Zebrafish (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa551-600 of human PDE8A (O60658, NP_002596). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig (100%), Mouse, Elephant, Pig, Opossum, Turkey, Zebra finch, Chicken, Xenopus, Zebrafish (92%), Marmoset, Bat (85%), Platypus, Drosophila (84%), Bloom alga (83%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Referenzen für anti-PDE8A Antikörper (ABIN6746531)

Shahan, Sorenson, Simpson, Kefalides, Lewis: "Tyrosine kinase activation in response to fungal spores is primarily dependent on endogenous reactive oxygen production in macrophages." in: The Journal of biological chemistry, Vol. 275, Issue 14, pp. 10175-81, (2000) (PubMed).

Yang, Malek, Desiderio: "An SH3-binding site conserved in Bruton's tyrosine kinase and related tyrosine kinases mediates specific protein interactions in vitro and in vivo." in: The Journal of biological chemistry, Vol. 270, Issue 35, pp. 20832-40, (1995) (PubMed).

Aoki, Isselbacher, Pillai: "Bruton tyrosine kinase is tyrosine phosphorylated and activated in pre-B lymphocytes and receptor-ligated B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 22, pp. 10606-9, (1994) (PubMed).

Vetrie, Vorechovský, Sideras, Holland, Davies, Flinter, Hammarström, Kinnon, Levinsky, Bobrow: "The gene involved in X-linked agammaglobulinaemia is a member of the src family of protein-tyrosine kinases." in: Nature, Vol. 361, Issue 6409, pp. 226-33, (1993) (PubMed).

Details zu PDE8A

Target: PDE8A (phosphodiesterase 8A (PDE8A))

Andere Bezeichnung: PDE8A (PDE8A Produkte)

Hintergrund: Name/Gene ID: PDE8A
Subfamily: PDE - cAMP-specific, high affinity
Family: Phosphodiesterase

Synonyms: PDE8A, Phosphodiesterase 8A, HsT19550

Gen-ID: 5151

NCBI Accession: NP_002596

UniProt: O60658

Pathways: cAMP Metabolic Process