Hexokinase 2 Antikörper (AA 74-123)
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- Target Alle Hexokinase 2 (HK2) Antikörper anzeigen
- Hexokinase 2 (HK2)
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Bindungsspezifität
- AA 74-123
- Reaktivität
- Human, Maus, Ratte, Schwein, Hund, Pferd, Kaninchen, Rind (Kuh), Affe, Meerschweinchen, Zebrafisch (Danio rerio), Hamster, Fledermaus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Hexokinase 2 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human HK2 / Hexokinase 2
- Homologie
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Chicken, Zebrafish (85%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa74-123 of human HK2 (P52789, NP_000180). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Sablefish, Stickleback (92%), Orangutan, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Zebrafish, Sea squirt (85%).
Type of Immunogen: Synthetic peptide - Isotyp
- IgG
- Top Product
- Discover our top product HK2 Primärantikörper
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- Applikationshinweise
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Distilled Water.
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- Hexokinase 2 (HK2)
- Andere Bezeichnung
- HK2 / Hexokinase 2 (HK2 Produkte)
- Synonyme
- HKII antikoerper, HXK2 antikoerper, AI642394 antikoerper, hexokinase-2 antikoerper, fi09d05 antikoerper, fi17h06 antikoerper, wu:fi09d05 antikoerper, wu:fi17h06 antikoerper, wu:fi31b04 antikoerper, zgc:55926 antikoerper, HK2 antikoerper, ARABIDOPSIS THALIANA HEXOKINASE 2 antikoerper, ATHXK2 antikoerper, F6F22.11 antikoerper, F6F22_11 antikoerper, HEXOKINASE 2 antikoerper, hexokinase 2 antikoerper, StHK2 antikoerper, hexokinase 2 antikoerper, hexokinase-2 antikoerper, hexokinase 2 L homeolog antikoerper, HK2 antikoerper, Hk2 antikoerper, hk2 antikoerper, HXK2 antikoerper, LOC100032849 antikoerper, hk2.L antikoerper, hxk2 antikoerper, LOC102577690 antikoerper
- Hintergrund
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Name/Gene ID: HK2
Subfamily: Hexokinase
Family: Non-protein Kinase
Synonyms: HK2, Hexokinase II, Hexokinase type II, Hexokinase-2, muscle, HK II, HXK2, Hexokinase-2, Muscle form hexokinase, Hexokinase 2, HKII, Hxk 2 - Gen-ID
- 3099
- NCBI Accession
- NP_000180
- UniProt
- P52789
- Pathways
- PI3K-Akt Signalweg, Carbohydrate Homeostasis, Warburg Effekt
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