RHOT1 Antikörper (N-Term)

Produktnummer ABIN6749728

Produktdetails anti-RHOT1 Antikörper

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte, Kaninchen, Rind (Kuh), Meerschweinchen, Pferd, Fledermaus, Huhn, Affe, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RHOT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human RHOT1

Homologie: Percent identity by BLAST analysis: Rat, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Xenopus (92%) Dog (82%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human RHOT1 (Q8IXI2, NP_060777). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Seabass, Stickleback (100%), Panda, Dog, Xenopus, Zebrafish, Drosophila (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 - 10 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu RHOT1

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Andere Bezeichnung: RHOT1 (RHOT1 Produkte)

Hintergrund: Name/Gene ID: RHOT1
Family: Ras GTPase superfamily IPR001806

Synonyms: RHOT1, ARHT1, HMiro-1, MIRO-1, Mitochondrial Rho GTPase 1, MIRO1, Mitochondrial Rho 1, Ras homolog family member T1

Gen-ID: 55288

NCBI Accession: NP_060777

UniProt: Q8IXI2