LIM Domain Kinase 1 Antikörper (AA 451-550)

Produktnummer ABIN680518

Produktdetails anti-LIM Domain Kinase 1 Antikörper

Target: LIM Domain Kinase 1 (LIMK1)

Bindungsspezifität: AA 451-550

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIM Domain Kinase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Cow,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LIMK1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-LIM Domain Kinase 1 Antikörper (ABIN680518)

Zhou, Liu, Luo, Zhou, Xia, Chen, Zhou, Zou, Cao, Cao: "MiR-20a inhibits cutaneous squamous cell carcinoma metastasis and proliferation by directly targeting LIMK1." in: Cancer biology & therapy, Vol. 15, Issue 10, pp. 1340-9, (2015) (PubMed).

Details zu LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Andere Bezeichnung: LIMK1 (LIMK1 Produkte)

Synonyme: limk1 antikoerper, xlimk1 antikoerper, GB16654 antikoerper, LIMK1 antikoerper, LIMK antikoerper, LIMK-1 antikoerper, LIM domain kinase 1 antikoerper, LIM domain kinase 1a antikoerper, LIM domain kinase 1 L homeolog antikoerper, LIM-domain containing, protein kinase antikoerper, LIMK1 antikoerper, limk1a antikoerper, limk1 antikoerper, CpipJ_CPIJ000717 antikoerper, LOC413152 antikoerper, Limk1 antikoerper, limk1.L antikoerper

Hintergrund:

Synonyms: LIMK, LIMK-1, LIM domain kinase 1, LIMK1

Background: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).

Gen-ID: 3984

UniProt: P53667

Pathways: Caspase Kaskade in der Apoptose, Regulation of Cell Size, CXCR4-mediated Signaling Events