PRKDC Antikörper (AA 151-300)

Produktnummer ABIN685417

Produktdetails anti-PRKDC Antikörper

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Bindungsspezifität: AA 151-300

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKDC Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Human, Ratte

Homologie: Mouse,Dog,Cow,Horse,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DNA PKcs

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Andere Bezeichnung: DNAPK/PRKDC (PRKDC Produkte)

Synonyme: DNA-PKcs antikoerper, DNAPK antikoerper, DNPK1 antikoerper, HYRC antikoerper, HYRC1 antikoerper, XRCC7 antikoerper, p350 antikoerper, DNA-PKCS antikoerper, dna-pkcs antikoerper, dnapk antikoerper, dnpk1 antikoerper, hyrc antikoerper, hyrc1 antikoerper, prkdc-A antikoerper, xrcc7 antikoerper, PRKDC antikoerper, AI326420 antikoerper, AU019811 antikoerper, DNAPDcs antikoerper, DOXNPH antikoerper, dxnph antikoerper, p460 antikoerper, scid antikoerper, slip antikoerper, protein kinase, DNA-activated, catalytic polypeptide antikoerper, protein kinase, DNA-activated, catalytic polypeptide L homeolog antikoerper, DNA-dependent protein kinase catalytic subunit antikoerper, protein kinase, DNA activated, catalytic polypeptide antikoerper, PRKDC antikoerper, prkdc.L antikoerper, LOC100633457 antikoerper, Prkdc antikoerper

Hintergrund:

Synonyms: HYRC, p350, DNAPK, DNPK1, HYRC1, IMD26, XRCC7, DNA-PKcs, DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, p460, PRKDC

Background: Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2, negatively regulates CRY1 phosphorylation.

Gen-ID: 5591

UniProt: P78527

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response