ACTA1/ACTA2A/ACTG2 Antikörper

Produktnummer ABIN6936350

Produktdetails anti-ACTA1/ACTA2A/ACTG2 Antikörper

Target: ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACTA1/ACTA2A/ACTG2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Kreuzreaktivität (Details): Human. Rabbit. Cat. Dog. Mouse. Rat. Chicken.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: SDS extract of human myocardium.

Klon: HHF35

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Muscle or sarcoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu ACTA1/ACTA2A/ACTG2

Target: ACTA1/ACTA2A/ACTG2 (ACTA1 (Skeletal), ACTA2 (Smooth), ACTG2 (Smooth))

Abstract: ACTA1/ACTA2A/ACTG2 Produkte

Hintergrund: ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1,Actin, Muscle Specific (Muscle Cell Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: Multiple

Gen-ID: 58, 59, 70

UniProt: P62736, P68032, P68133