CEACAM1/5 Antikörper

Produktnummer ABIN6936928

Produktdetails anti-CEACAM1/5 Antikörper

Target: CEACAM1/5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/5 (CEACAM1/5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CEACAM1/5 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)

Spezifität: This antibody recognizes proteins of 80-200 kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human colon carcinoma extract

Klon: COL-1

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF7 or 293T cells. Human colon carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CEACAM1/5

Target: CEACAM1/5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/5 (CEACAM1/5))

Andere Bezeichnung: CEACAM1 & CEACAM5 (CEACAM1/5 Produkte)

Synonyme: CD66e antikoerper, CEA antikoerper, carcinoembryonic antigen related cell adhesion molecule 5 antikoerper, CEACAM5 antikoerper

Hintergrund: Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1),Carcinoembryonic Antigen (CEA) / CD66
Cellular localisation: Cytoplasmic and luminal surface

Molekulargewicht: 80-200kDa

Gen-ID: 1048, 709196

UniProt: P06731