Chp2 Antikörper

Produktnummer ABIN6936993

Produktdetails anti-Chp2 Antikörper

Target: Chp2

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Chp2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: CHP2 (calcineurin B homologous protein 2), also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length CHP2 protein

Klon: CPTC-CHP2-1

Isotyp: IgG2c kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, K562 or Jurkat cell lysates. Human appendix or testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Chp2

Target: Chp2

Abstract: Chp2 Produkte

Synonyme: 2010110P09Rik antikoerper, Cbhp2 antikoerper, chp-2 antikoerper, calcineurin-like EF hand protein 2 antikoerper, calcineurin like EF-hand protein 2 antikoerper, Chp2 antikoerper, CHP2 antikoerper

Hintergrund: Calcineurin-like EF hand protein 2, calcineurin-like EF-hand protein 2, HCA520, hepatocellular carcinoma antigen gene 520, Hepatocellular carcinoma-associated antigen 520 homolog,Calcineurin B homologous protein 2 / HCC Antigen 520
Cellular localisation: Nucleus. Cytoplasm.

Molekulargewicht: 22kDa

Gen-ID: 63928, 178589

UniProt: O43745