toxA Antikörper

Produktnummer ABIN6937000

Produktdetails anti-toxA Antikörper

Target: toxA (Clostridium Difficile A Toxin (toxA))

Reaktivität: Clostridium difficile

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser toxA Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Coating (Coat)

Spezifität: This MAb reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.

Aufreinigung: Purified by Protein A/G

Immunogen: C. difficile Toxin A

Klon: 253-422

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Clostridium difficile extract or infected cells. Tissue.

Known Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA),Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM PBS without BSA and without Azide.

Konservierungsmittel: Azide free

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Haltbarkeit: 24 months

Details zu toxA

Target: toxA (Clostridium Difficile A Toxin (toxA))

Andere Bezeichnung: Clostridium difficile (Toxin A) (toxA Produkte)

Molekulargewicht: 308kDa (Toxin A)

UniProt: P16154