Desmocollin 2 Antikörper (Extracellular Domain)

Produktnummer ABIN6937104

Produktdetails anti-Desmocollin 2 Antikörper

Target: Desmocollin 2 (DSC2)

Bindungsspezifität: Extracellular Domain

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmocollin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. And desmocollin-3 is expressed more evenly throughout the suprabasal layers.

Kreuzreaktivität (Details): Human. Mouse. Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human desmocollin-2 extracellular domain

Klon: 7G6

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Skin tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Desmocollin 2

Target: Desmocollin 2 (DSC2)

Andere Bezeichnung: DSC2 (DSC2 Produkte)

Synonyme: ARVD11 antikoerper, CDHF2 antikoerper, DG2 antikoerper, DGII/III antikoerper, DSC3 antikoerper, AW228162 antikoerper, Dsc2a antikoerper, Dsc2b antikoerper, DSC2 antikoerper, desmocollin 2 antikoerper, DSC2 antikoerper, Dsc2 antikoerper

Hintergrund: ARVD11, Cadherin family member 2, CDHF2, Desmocollin-2, Desmocollin-3, Desmosomal glycoprotein II and III, Desmosomal glycoprotein II, Desmosomal glycoprotein III, DG2, DGII/III, DSC2, DSC3,Desmocollin-2/3
Cellular localisation: Cell surface

Molekulargewicht: 110kDa (DSC2), 100kDa (DSC3)

Gen-ID: 1824, 95612

UniProt: Q02487