Rekombinanter MHC Class II HLA-DP/DQ/DR Antikörper
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- Target Alle MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR) Produkte
- MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
- Antikörpertyp
- Recombinant Antibody
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser MHC Class II HLA-DP/DQ/DR Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36 kDa) and a beta chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.
- Kreuzreaktivität (Details)
- Human.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Non-T, non-B human acute lymphoblastic leukemia REH6 cells
- Klon
- RHLA-Pan-3475
- Isotyp
- IgG1 kappa
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- Applikationshinweise
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Raji cells. Human tonsil or lymph node.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
- Andere Bezeichnung
- HLA-DP, HLA-DQ, HLA-DR (HLA-DP/DQ/DR Produkte)
- Hintergrund
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HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5,HLA-Pan (MHC II)
Cellular localisation: Cell Surface - Molekulargewicht
- 33-35kDa
- UniProt
- P01908, P01909
- Pathways
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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