MHC Class II HLA-DP/DQ/DR Antikörper

Produktnummer ABIN6937400

Produktdetails anti-MHC Class II HLA-DP/DQ/DR Antikörper

Target: MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MHC Class II HLA-DP/DQ/DR Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Coating (Coat), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an chain (36 kDa) and a chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six and ten chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Cells from human tonsil

Klon: CR3-43

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating use Ab at 2-4 μg/mL, order Ab without BSA),Flow Cytometry (1-2 μg/ million cells), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Ramos, Raji cells. Tonsil, Spleen or lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu MHC Class II HLA-DP/DQ/DR

Target: MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)

Andere Bezeichnung: HLA-DP, HLA-DQ, HLA-DR (HLA-DP/DQ/DR Produkte)

Hintergrund: HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5,HLA-DP/-DQ/-DR (MHC II)
Cellular localisation: Cell Surface

Molekulargewicht: 36kDa (' chain) and 27kDa (' chain)

Gen-ID: 3115, 3117, 3122

UniProt: P04440, P01908, P01909, P01920, P01903

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response