NKX6-1 Antikörper

Produktnummer ABIN6938077

Produktdetails anti-NKX6-1 Antikörper

Target: NKX6-1 (NK6 Homeobox 1 (NKX6-1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NKX6-1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Members of the Nkx family of homeodomain proteins are key regulators of growth and development in several tissues, including brain, heart and pancreas. During neural development, sonic hedgehog (Shh) is known to control cell fate and mitogenesis, which is correlated with Shh dose-dependent expression of several genes, including Nkx-6.1. Specifically, Nkx-6.1 is responsible for cellular differentiation in the ventral neural tube and spinal meninges in response to Shh. In the pancreas, Nkx-6.1 is exclusively expressed in the islets of Langerhans in differentiating and mature B cells, which produce Insulin. The presence of Pdx-1 is required for the expression of Nkx-6.1 as well as other pancreatic B cell specific genes, including Insulin, Glut2 and IAPP. Subsequently, Nkx-6.1 binds to the DNA consensus sequence, TTAATTAC, to direct the repression of specific genes in B cells. Nkx6.1 is highly expressed in pancreatic and duodenal well-differentiated neuroendocrine tumors (WDNETS) and in metastatic WDNETS, is a highly specific marker of tumors of pancreatic origin. It has thus been suggested that Nkx6.1 is a useful inclusion into IHC panels for identifying primary sites of WDNETS.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human NKX6.1 protein

Klon: NKX61-2561

Isotyp: IgG2c kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Human fetal small intestine lysate or pancreas tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu NKX6-1

Target: NKX6-1 (NK6 Homeobox 1 (NKX6-1))

Andere Bezeichnung: NKX6-1 (NKX6-1 Produkte)

Synonyme: nkx6a antikoerper, nkx6.1 antikoerper, NKX6.1 antikoerper, NKX6A antikoerper, zgc:92879 antikoerper, Nkx6.1 antikoerper, Nkx61 antikoerper, Nkx6a antikoerper, NK6 homeobox 1 antikoerper, NK6 homeobox 1 L homeolog antikoerper, nkx6-1 antikoerper, NKX6-1 antikoerper, nkx6.1 antikoerper, nkx6-1.L antikoerper, Nkx6-1 antikoerper

Hintergrund: Homeobox protein NK-6 homolog A, NK homeobox, family 6, A, NK6 homeobox 1, NK6 transcription factor homolog A, NK6 transcription factor related, locus 1 (Drosophila), NKX6-1, NKX6A,NKX6.1 (Marker for Pancreatic and Duodenal Neuroendocrine Tumors)
Cellular localisation: Nuclear

Molekulargewicht: 40-50kDa

Gen-ID: 4825, 546270

UniProt: P78426

Pathways: Dopaminergic Neurogenesis, Positive Regulation of Peptide Hormone Secretion