SPI1 Antikörper (AA 16-170)
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- Target Alle SPI1 Antikörper anzeigen
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
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Bindungsspezifität
- AA 16-170
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser SPI1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.
- Kreuzreaktivität (Details)
- Human.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary)
- Klon
- PU1-2146
- Isotyp
- IgG2b kappa
- Top Product
- Discover our top product SPI1 Primärantikörper
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- Applikationshinweise
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Western Blot (1-2 μg/mL),Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Ramos, K562 or THP-1 cells. Lymph Node, Spleen, Hodgkin s Lymphoma, Colon Carcinoma.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))
- Andere Bezeichnung
- SPI1 (SPI1 Produkte)
- Synonyme
- OF antikoerper, PU.1 antikoerper, SFPI1 antikoerper, SPI-1 antikoerper, SPI-A antikoerper, Dis-1 antikoerper, Dis1 antikoerper, Sfpi-1 antikoerper, Sfpi1 antikoerper, Spi-1 antikoerper, Tcfpu1 antikoerper, Tfpu.1 antikoerper, Pu.1 antikoerper, cb1086 antikoerper, pu.1 antikoerper, pu1 antikoerper, sfpi1 antikoerper, si:by184l24.2 antikoerper, spi1 antikoerper, SPI1/PU.1 antikoerper, spi1l antikoerper, zgc:152991 antikoerper, Spi-1 proto-oncogene antikoerper, spleen focus forming virus (SFFV) proviral integration oncogene antikoerper, Spi-1 proto-oncogene b antikoerper, Spi-1 proto-oncogene a antikoerper, SPI1 antikoerper, Spi1 antikoerper, spi1b antikoerper, spi1a antikoerper
- Hintergrund
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Transcription Factor spi1, 31 kDa Transforming Protein, Hematopoietic Transcription Factor PU.1, SFPI1, SPI1, SPIA, Spleen focus forming virus (SFFV) proviral integration oncogene spi1,PU.1 (SPI-1) (B-Cell Marker)
Cellular localisation: Nuclear - Molekulargewicht
- 40kDa
- Gen-ID
- 6688, 502511
- UniProt
- P17947
- Pathways
- Stem Cell Maintenance
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