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TOP1MT Antikörper

TOP1MT Reaktivität: Human IF, WB, FACS, IHC (f) Wirt: Maus Monoclonal TOP1MT-488 unconjugated
Produktnummer ABIN6938633
  • Target Alle TOP1MT Antikörper anzeigen
    TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
    Reaktivität
    • 28
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 19
    • 11
    Maus
    Klonalität
    • 17
    • 13
    Monoklonal
    Konjugat
    • 14
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser TOP1MT Antikörper ist unkonjugiert
    Applikation
    • 10
    • 9
    • 9
    • 9
    • 7
    • 4
    • 3
    • 2
    Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Spezifität
    DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
    Kreuzreaktivität (Details)
    Human.
    Aufreinigung
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogen
    Recombinant full-length human TOP1MT protein
    Klon
    TOP1MT-488
    Isotyp
    IgG2b kappa
    Top Product
    Discover our top product TOP1MT Primärantikörper
  • Applikationshinweise

    Known_Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: MCF-7 or A431 cells. Jurkat cell lysate. Human heart, skeletal muscle, brain or fetal liver tissue.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Konservierungsmittel
    Azide free
    Lagerung
    -20 °C,-80 °C
    Informationen zur Lagerung
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Haltbarkeit
    24 months
  • Target
    TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
    Andere Bezeichnung
    TOP1MT (TOP1MT Produkte)
    Synonyme
    2900052H09Rik antikoerper, TOP1 antikoerper, topoisomerase (DNA) I, mitochondrial antikoerper, DNA topoisomerase 1, mitochondrial antikoerper, DNA topoisomerase I mitochondrial antikoerper, TOP1MT antikoerper, Top1mt antikoerper
    Hintergrund
    DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase,Topoisomerase I, Mitochondrial (TOP1MT)
    Cellular localisation: Cytoplasm (Mitochondria)
    Molekulargewicht
    70kDa
    Gen-ID
    116447, 528574
    UniProt
    Q969P6
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