TOP1MT Antikörper
-
- Target Alle TOP1MT Antikörper anzeigen
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
-
Reaktivität
- Human
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser TOP1MT Antikörper ist unkonjugiert
-
Applikation
- Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
- Kreuzreaktivität (Details)
- Human.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human TOP1MT protein
- Klon
- TOP1MT-488
- Isotyp
- IgG2b kappa
- Top Product
- Discover our top product TOP1MT Primärantikörper
-
-
- Applikationshinweise
-
Known_Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7 or A431 cells. Jurkat cell lysate. Human heart, skeletal muscle, brain or fetal liver tissue.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
-
- Target
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
- Andere Bezeichnung
- TOP1MT (TOP1MT Produkte)
- Synonyme
- 2900052H09Rik antikoerper, TOP1 antikoerper, topoisomerase (DNA) I, mitochondrial antikoerper, DNA topoisomerase 1, mitochondrial antikoerper, DNA topoisomerase I mitochondrial antikoerper, TOP1MT antikoerper, Top1mt antikoerper
- Hintergrund
-
DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase,Topoisomerase I, Mitochondrial (TOP1MT)
Cellular localisation: Cytoplasm (Mitochondria) - Molekulargewicht
- 70kDa
- Gen-ID
- 116447, 528574
- UniProt
- Q969P6
-