TRAF1 Antikörper (AA 73-219)

Produktnummer ABIN6938672

Produktdetails anti-TRAF1 Antikörper

Target: TRAF1 (TNF Receptor-Associated Factor 1 (TRAF1))

Bindungsspezifität: AA 73-219

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TRAF1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: This MAb recognizes a protein of 52 kDa, which is identified as TNFR1 (TNFR-associated factor 1). CD30-positive lymphoproliferations of the skin comprise 30 % of all primary cutaneous T-cell lymphomas (CTCLs). Besides borderline cases this group includes lymphomatoid papulosis (LyP) and primary cutaneous anaplastic large T-cell lymphoma (cALCL). Although the two entities overlap clinically, histopathologically, immunopathologically and genetically, they differ considerably in their prognosis. In particular, common feature of both cases is histologically the presence of atypical lymphoid CD30-positive T blasts and genetically a clonal T-cell-receptor rearrangement. However, both cases differ considerably in their clinical course: Lesions of LyP regress spontaneously, whereas those of cALCL persist and may progress and spread. Moreover, LyP patients do not benefit from an aggressive radio- and/or chemotherapeutic approach, in contrast to patients with cALCL. Besides, LyP and cALCL differ strongly in the expression of TRAF1 (tumor necrosis factor receptor (TNFR)-associated factor 1), a component of TNFR signaling: Whereas tumor cells of most LyP cases (ca. 84 %) show a strong TRAF1 expression, tumor cells of cALCL reveal TRAF1 expression in only a few cases (ca. 7 %). Antibody to TRAF1 is highly useful for the differentiation of LyP and cALCL in patients with cutaneous CD30-positive lymphoproliferations.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human TRAF1 protein (around aa 73-219) (exact sequence is proprietary)

Klon: TRAF1-2770

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or 293T cells. Hodgkin s Lymphoma (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu TRAF1

Target: TRAF1 (TNF Receptor-Associated Factor 1 (TRAF1))

Andere Bezeichnung: TRAF1 (TRAF1 Produkte)

Synonyme: CG3048 antikoerper, DTRAF antikoerper, DTRAF1 antikoerper, Dmel\\CG3048 antikoerper, TRAF antikoerper, TRAF1 antikoerper, Traf1 antikoerper, dTRAF antikoerper, dTRAF1 antikoerper, dTraf1 antikoerper, dtraf1 antikoerper, traf1 antikoerper, traf4 antikoerper, DKFZp469G032 antikoerper, EBI6 antikoerper, MGC:10353 antikoerper, 4732496E14Rik antikoerper, TNF receptor associated factor 1 antikoerper, TNF-receptor-associated factor 4 antikoerper, TNF receptor-associated factor 1 antikoerper, TRAF1 antikoerper, Traf4 antikoerper, Traf1 antikoerper, traf1 antikoerper

Hintergrund: EBI6, EBV induced protein 6, Epstein-Barr virus-induced protein 6, TNF receptor-associated factor 1, TRAF1,TRAF1 (TNFR-Associated Factor 1) (Lymphomatoid Papulosis Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 52kDa

Gen-ID: 7185, 531251

UniProt: Q13077

Pathways: NF-kappaB Signalweg, Apoptose, Cell-Cell Junction Organization, Asymmetric Protein Localization