Villin 1 Antikörper (AA 179-311)
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- Target Alle Villin 1 (VIL1) Antikörper anzeigen
- Villin 1 (VIL1)
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Bindungsspezifität
- AA 179-311
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Villin 1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
- Kreuzreaktivität (Details)
- Human.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary))
- Klon
- VIL1-1325
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product VIL1 Primärantikörper
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- Applikationshinweise
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Known_Application: Western Blot (1-2 μg/mL for 60 minutes at RT),Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: A549, HepG2 and HCT116 cells. Colon or Rectum.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- Villin 1 (VIL1)
- Andere Bezeichnung
- VIL1 (VIL1 Produkte)
- Synonyme
- D2S1471 antikoerper, VIL antikoerper, Vil antikoerper, vil1 antikoerper, MGC64339 antikoerper, VIL1 antikoerper, vil1l antikoerper, zgc:55779 antikoerper, villin 1 antikoerper, villin 1 S homeolog antikoerper, VIL1 antikoerper, Vil1 antikoerper, vil1.S antikoerper, vil1 antikoerper
- Hintergrund
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VIL1, Villin-1, Villin1,Villin (GI-Mucosal & Urogenital Brush Border Marker)
Cellular localisation: Cytoplasmic & Cell Surface - Molekulargewicht
- 93kDa
- Gen-ID
- 7429, 654595
- UniProt
- P09327
- Pathways
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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