VISTA Antikörper
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- Target Alle VISTA Antikörper anzeigen
- VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser VISTA Antikörper ist unkonjugiert
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Applikation
- ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC s, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy.
- Kreuzreaktivität (Details)
- Human.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human VISTA protein
- Klon
- VISTA-3007
- Isotyp
- IgG2b kappa
- Top Product
- Discover our top product VISTA Primärantikörper
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- Applikationshinweise
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Known_Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Spleen or Placenta.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
- Andere Bezeichnung
- VSIR (VISTA Produkte)
- Synonyme
- MGC112715 antikoerper, MGC151567 antikoerper, B7-H5 antikoerper, B7H5 antikoerper, GI24 antikoerper, PP2135 antikoerper, SISP1 antikoerper, Dies1 antikoerper, VISTA antikoerper, V-set immunoregulatory receptor antikoerper, vsir antikoerper, Vsir antikoerper, VSIR antikoerper
- Hintergrund
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B7H5, DD1alpha, GI24, PD-1H, PDCD1 homolog, Platelet receptor Gi24, PP2135, Stress-induced secreted protein 1 (SISP1), V domain Ig suppressor of T cell activation (VISTA), V Set Immuno-Regulatory Receptor,VISTA / B7-H5 / VSIR (Negative Regulator of Immune Response)
Cellular localisation: Cell Surface and Cytoplasmic - Molekulargewicht
- 34kDa
- Gen-ID
- 64115, 47382
- UniProt
- Q9H7M9
- Pathways
- Cancer Immune Checkpoints
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