OLIG2 Antikörper (AA 1-141)

Produktnummer ABIN6939031

Produktdetails anti-OLIG2 Antikörper

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Bindungsspezifität: AA 1-141

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser OLIG2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Olig2, a basic helix-loop-helix transcription factor, is involved in oligodendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuro-epithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid / rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue (gliosis).

Kreuzreaktivität (Details): Human,

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human OLIG2 protein (around aa 1-141) (exact sequence is proprietary)

Klon: OLIG2-2400

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Positive Control: THP-1 cells. Astrocytoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu OLIG2

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Andere Bezeichnung: OLIG2 (OLIG2 Produkte)

Synonyme: olig3.2 antikoerper, MGC89336 antikoerper, OLIG2 antikoerper, BHLHB1 antikoerper, OLIGO2 antikoerper, PRKCBP2 antikoerper, RACK17 antikoerper, bHLHe19 antikoerper, wu:fc64g09 antikoerper, AI604895 antikoerper, Bhlhb1 antikoerper, Olg-2 antikoerper, Oligo2 antikoerper, RK17 antikoerper, oligodendrocyte transcription factor 4 antikoerper, oligodendrocyte transcription factor 2 antikoerper, oligodendrocyte lineage transcription factor 2 antikoerper, olig4 antikoerper, OLIG2 antikoerper, Olig2 antikoerper, olig2 antikoerper

Hintergrund: Basic helix loop helix protein class B1 (bHLHB1), basic helix-loop-helix protein 19 (bHLHe19), OLIG2, Oligodendrocyte lineage transcription factor 2, Oligodendrocyte specific bHLH transcription factor 2, Oligodendrocyte transcription factor 2, Protein kinase C-binding protein 2 (PRKCBP2), RACK17,OLIG2 (Marker of Glial Brain Tumors)
Cellular localisation: Nuclear

Molekulargewicht: 30-40kDa

Gen-ID: 10215, 176977

UniProt: Q13516