CDKN1C Antikörper

Produktnummer ABIN6939050

Produktdetails anti-CDKN1C Antikörper

Target: CDKN1C (Cyclin-Dependent Kinase Inhibitor 1C (p57, Kip2) (CDKN1C))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDKN1C Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Recognizes a protein of 57 kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.

Kreuzreaktivität (Details): Human, Mouse,

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human p57Kip2 protein

Klon: SPM308

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Positive Control: LS174T, Raji, HT29, SK-BR3 cells. Colon or Prostate carcinomas.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu CDKN1C

Target: CDKN1C (Cyclin-Dependent Kinase Inhibitor 1C (p57, Kip2) (CDKN1C))

Andere Bezeichnung: CDKN1C (CDKN1C Produkte)

Synonyme: BWCR antikoerper, BWS antikoerper, KIP2 antikoerper, WBS antikoerper, p57 antikoerper, p57Kip2 antikoerper, cb961 antikoerper, p27Xic1-a antikoerper, zgc:86618 antikoerper, AL024410 antikoerper, CDKI antikoerper, Kip2 antikoerper, p57(kip2) antikoerper, p57KIP2 antikoerper, cyclin dependent kinase inhibitor 1C antikoerper, cyclin-dependent kinase inhibitor 1Ca antikoerper, cyclin-dependent kinase inhibitor 1C (P57) antikoerper, cyclin-dependent kinase inhibitor 1C antikoerper, CDKN1C antikoerper, cdkn1ca antikoerper, Cdkn1c antikoerper

Hintergrund: Beckwith Wiedemann syndrome (WBS), BWCR, Cyclin dependent kinase inhibitor 1C (CDKN1C), KIP2, p57, p57Kip2,p57Kip2 (Mitotic Inhibitor/Suppressor Protein)
Cellular localisation: Nuclear

Molekulargewicht: 57kDa

Gen-ID: 1028, 106070

UniProt: P49918

Pathways: Zellzyklus, Dopaminergic Neurogenesis