Nestin Antikörper (AA 698-844)

Produktnummer ABIN6939087

Produktdetails anti-Nestin Antikörper

Target: Nestin (NES)

Bindungsspezifität: AA 698-844

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nestin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or -internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differentiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.

Kreuzreaktivität (Details): Human,

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human NES protein fragment around aa 698-844 (exact sequence is proprietary)

Klon: NES-2911

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: Human kidney, ovary, cervix, breast, colon, endometrium, cerebellum, lung, prostate, skin and human melanoma tissue. HeLa cell lysate. SH-SY5Y cells.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu Nestin

Target: Nestin (NES)

Andere Bezeichnung: NES (NES Produkte)

Synonyme: paranemin antikoerper, NES antikoerper, AA166324 antikoerper, C78523 antikoerper, ESTM46 antikoerper, nestin antikoerper, nestin S homeolog antikoerper, NES antikoerper, nes.S antikoerper, Nes antikoerper, nes antikoerper

Hintergrund: ESTM 46, FLJ21841, Intermediate filament protein, Nbla00170, nes,Nestin (Cancer Stem Cell Marker)
Cellular localisation: Cytoplasm and membrane.

Molekulargewicht: 190-200kDa

Gen-ID: 10763, 527971

UniProt: P48681