CLTA/CLTB Antikörper (N-Term)

Produktnummer ABIN6939151

Produktdetails anti-CLTA/CLTB Antikörper

Target: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CLTA/CLTB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Spezifität: Recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.

Aufreinigung: Purified by Protein A/G

Immunogen: Purified recombinant N-terminal fragment of human Clathrin Light Chain

Klon: SPM174

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Placenta or Prostate Carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CLTA/CLTB

Target: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Andere Bezeichnung: CLTA and CLTB (CLTA/CLTB Produkte)

Synonyme: lca antikoerper, zgc:73358 antikoerper, wu:fb61d08 antikoerper, wu:fj48d06 antikoerper, LCA antikoerper, clc antikoerper, DDBDRAFT_0169251 antikoerper, DDBDRAFT_0191305 antikoerper, DDB_0169251 antikoerper, DDB_0191305 antikoerper, clcb antikoerper, clathrin light chain A antikoerper, clathrin, light chain A antikoerper, Clathrin light chain antikoerper, clathrin light chain antikoerper, clathrin light chain B antikoerper, Clathrin light chain B antikoerper, CLTA antikoerper, clta antikoerper, clic-1 antikoerper, CLC1 antikoerper, clc antikoerper, CLTB antikoerper, clcb antikoerper, CNI00580 antikoerper, LOC5567495 antikoerper, CpipJ_CPIJ018056 antikoerper, PTRG_01988 antikoerper, PMAA_096950 antikoerper, SJAG_04517 antikoerper, TSTA_044110 antikoerper, PAS_chr1-4_0158 antikoerper

Molekulargewicht: 31-44kDa

Gen-ID: 1211, 1212

UniProt: P09496, P09497