CD35 Antikörper

Produktnummer ABIN6939177

Produktdetails anti-CD35 Antikörper

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reaktivität: Human, Pavian, Cynomolgus, Rhesusaffen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD35 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Spezifität: Recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This MAb is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein.Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.

Aufreinigung: Purified by Protein A/G

Immunogen: Intact human monocytes

Klon: E11

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Follicular dendritic cells (FDC) in tonsil.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Andere Bezeichnung: CR1 (CR1 Produkte)

Synonyme: C3BR antikoerper, C4BR antikoerper, CD35 antikoerper, KN antikoerper, C3DR antikoerper, CD21 antikoerper, Cr-1 antikoerper, Cr-2 antikoerper, Cr1 antikoerper, CR1-L antikoerper, CR2 antikoerper, CR1 antikoerper, complement C3b/C4b receptor 1 (Knops blood group) antikoerper, complement receptor 2 antikoerper, complement component (3d/Epstein Barr virus) receptor 2 antikoerper, complement receptor type 1 antikoerper, CR1 antikoerper, Cr2 antikoerper, CR2 antikoerper, LOC718120 antikoerper

Molekulargewicht: 210-220kDa

Gen-ID: 1378

UniProt: P17927

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity