MLANA Antikörper

Produktnummer ABIN6939392

Produktdetails anti-MLANA Antikörper

Target: MLANA (Melan A (MLANA))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MLANA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Spezifität: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant hMART-1 protein

Klon: M2-9E3

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MLANA

Target: MLANA (Melan A (MLANA))

Andere Bezeichnung: MLANA (MLANA Produkte)

Synonyme: A930034P04Rik antikoerper, Mart1 antikoerper, MART-1 antikoerper, MART1 antikoerper, melan-A antikoerper, Mlana antikoerper, MLANA antikoerper

Molekulargewicht: 20-22kDa (doublet)

Gen-ID: 2315

UniProt: Q16655