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EpCAM Antikörper (Extracellular Domain)

Name: EpCAM Antikörper (Extracellular Domain)
Brand: antibodies-online
SKU: ABIN6939978
Price: 663.89 EUR
Availability: InStock

EPCAM Reaktivität: Human WB, FACS, IF, IHC, StM Wirt: Maus Monoclonal EGP40-1373 unconjugated

Formalin-fixed, paraffin-embedded feline (cat) small intestine stained with Ep-CAM Mouse Monoclonal Antibody (EGP40/1373). (EpCAM Antikörper (Extracellular Domain))

IHC

Analysis of Protein Array containing >19,000 full-length human proteins using EpCAM Mouse Monoclonal Antibody (EGP40/1373) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (EpCAM Antikörper (Extracellular Domain))

PAr

Confocal Immunofluorescence of MCF-7 cells EpCAM Mouse Monoclonal Antibody (EGP40/1373). (EpCAM Antikörper (Extracellular Domain))

Formalin-fixed, paraffin-embedded human FallopianTube stained with EpCAM Mouse Monoclonal Antibody (EGP40/1373). (EpCAM Antikörper (Extracellular Domain))

IHC

SDS-PAGE Analysis Purified EpCAM Mouse Monoclonal Antibody (EGP40/1373). (EpCAM Antikörper (Extracellular Domain))

SDS

Western Blot Analysis of human lung lysate using EpCAM Mouse Monoclonal Antibody (EGP40/1373). (EpCAM Antikörper (Extracellular Domain))

Formalin-fixed, paraffin-embedded canine (dog) colon stained with Ep-CAM Mouse Monoclonal Antibody (EGP40/1373). (EpCAM Antikörper (Extracellular Domain))

IHC

7 Abbildungen

Produktnummer ABIN6939978

Datenblatt (PDF)

Target Alle EpCAM (EPCAM) Antikörper anzeigen

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
Bindungsspezifität
56

41

30

29

16

16

14

13

12

11

10

8

5

4

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 77-202, Extracellular Domain
Reaktivität
308

87

36

2

1

1
Human
Wirt
162

153

20

1
Maus
Passende Sekundärantikörper
Klonalität
215

117

2
Monoklonal
Konjugat
163

22

19

10

9

9

8

8

8

8

8

5

5

5

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
Dieser EpCAM Antikörper ist unkonjugiert
Applikation
220

192

124

116

90

38

30

26

24

21

16

16

15

11

4

4

3

2

2

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Aufreinigung

Purified by Protein A/G

Immunogen

Recombinant human EpCAM protein fragment from extracellular domain (around aa77-202) (exact sequence is proprietary)

Klon

EGP40-1373

Isotyp

IgG2b kappa

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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reaktivität: Human WB, IHC, StM Wirt: Maus Monoclonal rEGP40-1372 unconjugated Recombinant Antibody

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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM Reaktivität: Human WB, FACS, IF, IHC, StM Wirt: Maus Monoclonal EGP40-1372 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 20-60), (N-Term) antibody
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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 280-350), (Cytoplasmic Domain) antibody
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EPCAM Reaktivität: Human WB, FACS, IHC (p), IP, ICC Wirt: Maus Monoclonal 323-A3 unconjugated

Applikationshinweise

Positive Control: HT29 cells. Ovarian carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Western Blot (0.5-1.0 μg/mL), Immunofluorescence (1-3 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-80 °C

Informationen zur Lagerung

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit

24 months
Target

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung

TACSTD1 (EPCAM Produkte)

Hintergrund

EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Molekulargewicht

40-43kDa

Gen-ID

4072

UniProt

P16422