MUC1 Antikörper

Produktnummer ABIN6940096

Produktdetails anti-MUC1 Antikörper

Target: MUC1 (Mucin 1 (MUC1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat), Staining Methods (StM)

Spezifität: This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in 90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Aufreinigung: Purified by Protein A/G

Immunogen: Human milk-fat globule membranes (HMFGM)

Klon: MUC1-955

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

Known Application: ELISA (For coating use Ab at 2-5 μg/mL, order Ab without BSA) (Very good capturing Ab), Western Blot (0.5-2 μg/mL), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MUC1

Target: MUC1 (Mucin 1 (MUC1))

Andere Bezeichnung: MUC1 (MUC1 Produkte)

Synonyme: CA 15-3 antikoerper, CD227 antikoerper, EMA antikoerper, H23AG antikoerper, KL-6 antikoerper, MAM6 antikoerper, MCKD1 antikoerper, MUC-1 antikoerper, MUC-1/SEC antikoerper, MUC-1/X antikoerper, MUC1/ZD antikoerper, PEM antikoerper, PEMT antikoerper, PUM antikoerper, Muc-1 antikoerper, mucin 1, cell surface associated antikoerper, mucin 1, transmembrane antikoerper, MUC1 antikoerper, Muc1 antikoerper

Molekulargewicht: 265-400kDa

Gen-ID: 4582

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling