Nucleolin Antikörper

Produktnummer ABIN6940176

Produktdetails anti-Nucleolin Antikörper

Target: Nucleolin (NCL)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nucleolin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)

Spezifität: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This Mouse Monoclonal Antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Keine Kreuzreaktivität: Rind (Kuh), Maus, Ratte (Rattus)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human NCL protein

Klon: NCL-902

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa, Jurkat cells. All human cells. Testis, Ovary, Uterus, Endometrial or Hodgkin's lymphoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Nucleolin

Target: Nucleolin (NCL)

Andere Bezeichnung: NCL (NCL Produkte)

Synonyme: C23 antikoerper, B530004O11Rik antikoerper, D0Nds28 antikoerper, D1Nds28 antikoerper, Nucl antikoerper, wu:fa12d03 antikoerper, zgc:152810 antikoerper, nucl antikoerper, LOC100282923 antikoerper, LOC100283058 antikoerper, nucleolin antikoerper, nucleolin S homeolog antikoerper, NCL antikoerper, Ncl antikoerper, ncl.S antikoerper, ncl antikoerper, LOC100195537 antikoerper, LOC100282923 antikoerper, LOC100283058 antikoerper

Molekulargewicht: 76kDa

Gen-ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization