NEFH Antikörper

Produktnummer ABIN6940184

Dieses Anti-NEFH-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NEFH in WB, IHC, FACS und StM. Geeignet für Human, Ratte, Maus, Huhn, Schwein, Rind (Kuh), Kaninchen, Meerschweinchen, Wüstenrennmaus und Cat.

Kurzübersicht für NEFH Antikörper (ABIN6940184)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Human, Ratte, Maus, Huhn, Schwein, Rind (Kuh), Kaninchen, Meerschweinchen, Wüstenrennmaus, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Klon: NE14

Produktdetails anti-NEFH Antikörper

Spezifität: This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Aufreinigung: Purified by Protein A/G

Immunogen: Crude neurofilament preparation from porcine spinal cord

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HEK293 cells, Brain, Neuroblastoma.

Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (0.5-1 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: NEFH

Molekulargewicht: 200kDa

Gen-ID: 4744

UniProt: P12036