CD73 Antikörper
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- Target Alle CD73 (NT5E) Antikörper anzeigen
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD73 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Staining Methods (StM), Coating (Coat)
- Aufreinigung
- Purified by Protein A/G
- Immunogen
- Recombinant full-length human NT5E protein
- Klon
- NT5E-2646
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product NT5E Primärantikörper
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- Applikationshinweise
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Positive Control: A431, U87MG, PC-3 cells. Tonsil, Prostate or Lung Carcinoma.
Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells in 0.1ml),Immunofluorescence (1-2 μg/mL),Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 200 μg/mL
- Buffer
- 10 mM PBS with0.05 % BSA & 0.05 % azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Haltbarkeit
- 24 months
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- Target
- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
- Andere Bezeichnung
- NT5E (NT5E Produkte)
- Synonyme
- CALJA antikoerper, CD73 antikoerper, E5NT antikoerper, NT antikoerper, NT5 antikoerper, NTE antikoerper, eN antikoerper, eNT antikoerper, zgc:63784 antikoerper, NT5E antikoerper, MGC114869 antikoerper, PSPTO2277 antikoerper, 2210401F01Rik antikoerper, AI447961 antikoerper, Nt5 antikoerper, 5'-nucleotidase ecto antikoerper, 5'-nucleotidase, ecto (CD73) antikoerper, 5'-nucleotidase antikoerper, 5' nucleotidase, ecto antikoerper, NT5E antikoerper, nt5e antikoerper, nt5e.S antikoerper, PSPTO_2277 antikoerper, CBU_1233 antikoerper, MCA1322 antikoerper, Nt5e antikoerper
- Hintergrund
- CD73 is a membrane-bound extracellular enzyme overexpressed in several cancer types. Its expression has been linked to poor prognosis in melanoma, colorectal, gastric, triple negative breast cancer, and to a pro-metastatic phenotype in prostate cancer. Together with CD39, it plays a major role in promoting immunosuppression through the pathway degrading adenosine triphosphate (ATP) into adenosine. Within the tumor microenvironment, ATP promotes immune cell-mediated killing of cancer cells. In contrast, adenosine accumulation causes immune suppression, dysregulation of immune cell infiltrates and stimulates angiogenesis resulting in tumor spreading. CD73 is active on the last step of the degradation pathway, where it is the enzyme that actually degrades AMP into adenosine. CD73-blockade promotes anti-tumor immunity by reducing adenosine accumulation. Accordingly, anti-CD73 mAbs stimulate anti-tumor immunity and reduce tumor metastasis in Mouse tumor models, and could enhance the efficacy of treatment with anti-PD1 or anti-CTLA4 antibodies.
- Molekulargewicht
- 71kDa
- Gen-ID
- 4907
- UniProt
- P21589
- Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
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