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Cytochrome C Antikörper (AA 1-80)

CYCS Reaktivität: Human, Ratte, Maus, Pferd, Hund, Taube, Drosophila melanogaster, Frosch WB, IHC, IF, FACS, StM Wirt: Maus Monoclonal SPM389 unconjugated
Produktnummer ABIN6940320
  • Target Alle Cytochrome C (CYCS) Antikörper anzeigen
    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
    Bindungsspezifität
    • 16
    • 12
    • 10
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-80
    Reaktivität
    • 143
    • 115
    • 94
    • 42
    • 29
    • 22
    • 21
    • 19
    • 17
    • 15
    • 5
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Ratte, Maus, Pferd, Hund, Taube, Drosophila melanogaster, Frosch
    Wirt
    • 85
    • 73
    • 6
    • 1
    Maus
    Klonalität
    • 85
    • 79
    • 1
    Monoklonal
    Konjugat
    • 94
    • 13
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Cytochrome C Antikörper ist unkonjugiert
    Applikation
    • 125
    • 49
    • 37
    • 34
    • 30
    • 29
    • 27
    • 23
    • 14
    • 13
    • 13
    • 13
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
    Spezifität
    It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
    Aufreinigung
    Purified by Protein A/G
    Immunogen
    Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
    Klon
    SPM389
    Isotyp
    IgG2b kappa
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  • Applikationshinweise

    Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-80 °C
    Informationen zur Lagerung
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Haltbarkeit
    24 months
  • Target
    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
    Andere Bezeichnung
    CYCS (CYCS Produkte)
    Molekulargewicht
    15kDa
    Gen-ID
    54205
    UniProt
    P99999
    Pathways
    Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity
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