Cytochrome C Antikörper (AA 1-80)
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- Target Alle Cytochrome C (CYCS) Antikörper anzeigen
- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
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Bindungsspezifität
- AA 1-80
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Reaktivität
- Human, Ratte, Maus, Pferd, Hund, Taube, Drosophila melanogaster, Frosch
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Cytochrome C Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- Spezifität
- It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
- Aufreinigung
- Purified by Protein A/G
- Immunogen
- Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
- Klon
- SPM389
- Isotyp
- IgG2b kappa
- Top Product
- Discover our top product CYCS Primärantikörper
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- Applikationshinweise
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Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Haltbarkeit
- 24 months
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- Target
- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
- Andere Bezeichnung
- CYCS (CYCS Produkte)
- Molekulargewicht
- 15kDa
- Gen-ID
- 54205
- UniProt
- P99999
- Pathways
- Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity
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