POU2AF1 Antikörper (AA 148-255)

Produktnummer ABIN6940356

Produktdetails anti-POU2AF1 Antikörper

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Bindungsspezifität: AA 148-255

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser POU2AF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)

Klon: BOB1-2424

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Raji, Ramos cells. Spleen or Tonsil.

Known Application: Flow Cytometry (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu POU2AF1

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Andere Bezeichnung: POU2AF1 (POU2AF1 Produkte)

Synonyme: BOB1 antikoerper, OBF-1 antikoerper, OBF1 antikoerper, OCAB antikoerper, BOB-1 antikoerper, BOB.1 antikoerper, Bob-1 antikoerper, Bob1 antikoerper, OBF.1 antikoerper, OCA-B antikoerper, POU class 2 associating factor 1 antikoerper, POU domain, class 2, associating factor 1 antikoerper, POU2AF1 antikoerper, Pou2af1 antikoerper

Hintergrund: BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.

Molekulargewicht: 35kDa

Gen-ID: 5450

UniProt: Q16633