Rekombinanter BCL6 Antikörper (AA 256-389)

Produktnummer ABIN6940532

Produktdetails anti-BCL6 Antikörper

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: AA 256-389

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser BCL6 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Spezifität: Recognizes a protein of 95 kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ('LH') cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human bcl-6 protein fragment (aa256-389) (exact sequence is proprietary)

Klon: BCL6-2497R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: Raji or Ramos cells. Tonsil or Hodgkin's lymphoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu BCL6

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Andere Bezeichnung: BCL6 (BCL6 Produkte)

Synonyme: bcl5 antikoerper, laz3 antikoerper, bcl6a antikoerper, znf51 antikoerper, zbtb27 antikoerper, MGC132260 antikoerper, Bcl5 antikoerper, BCL5 antikoerper, BCL6A antikoerper, LAZ3 antikoerper, ZBTB27 antikoerper, ZNF51 antikoerper, B-cell CLL/lymphoma 6 L homeolog antikoerper, B-cell CLL/lymphoma 6 antikoerper, B cell leukemia/lymphoma 6 antikoerper, bcl6.L antikoerper, bcl6 antikoerper, BCL6 antikoerper, Bcl6 antikoerper

Molekulargewicht: 95kDa

Gen-ID: 604

UniProt: P41182

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus