Rekombinanter s100a4 Antikörper (AA 1-200)

Produktnummer ABIN6940544

Produktdetails anti-s100a4 Antikörper

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: AA 1-200

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser s100a4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)

Klon: RS100A4-1481

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa, A549 or A375 cells (IF/FACS). HeLa, A549, T98G or A375 cell lysate (WB). Human placenta tissue (IHC).

Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Andere Bezeichnung: S100A4 (S100A4 Produkte)

Synonyme: 18A2 antikoerper, 42A antikoerper, CAPL antikoerper, FSP1 antikoerper, MTS1 antikoerper, P9KA antikoerper, PEL98 antikoerper, 42a antikoerper, Capl antikoerper, FSp1 antikoerper, Mts1 antikoerper, PeL98 antikoerper, metastasin antikoerper, pk9a antikoerper, S100A4 antikoerper, P9ka antikoerper, RNP9KA antikoerper, S100 calcium binding protein A4 antikoerper, S100 calcium-binding protein A4 antikoerper, S100A4 antikoerper, S100a4 antikoerper

Hintergrund: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Molekulargewicht: 10-12kDa

Gen-ID: 6275

UniProt: P26447

Pathways: S100 Proteine