S100A8 Antikörper

Produktnummer ABIN6940546

Dieses Maus Monoklonal-Antikörper erkennt spezifisch S100A8 in IHC, IF, FACS und StM. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Schwein, Kaninchen, Hund, Cat, Affe, Pferd, Meerschweinchen, Pavian und Ziege.

Kurzübersicht für S100A8 Antikörper (ABIN6940546)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Kaninchen, Hund, Cat, Affe, Pferd, Meerschweinchen, Pavian, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A8 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon: MRP8 7C12-4

Produktdetails anti-S100A8 Antikörper

Spezifität: Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12 kDa alpha chain and a 14 kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Aufreinigung: Purified by Protein A/G

Immunogen: Affinity Purified monocyte membrane preparation

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Tonsil, lymph node or spleen.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Andere Bezeichnung: S100A8

Molekulargewicht: MAC387

Gen-ID: 6279, 6280

UniProt: P05109, P06702

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine