Melanoma gp100 Antikörper
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- Target Alle Melanoma gp100 (PMEL) Antikörper anzeigen
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Melanoma gp100 Antikörper ist unkonjugiert
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Applikation
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)
- Immunogen
- Recombinant full-length human SILV protein
- Isotyp
- IgG
- Top Product
- Discover our top product PMEL Primärantikörper
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- Applikationshinweise
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Positive Control: SK-MEL-28 cells. Melanoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Haltbarkeit
- 24 months
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- Target
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
- Andere Bezeichnung
- SILV (PMEL Produkte)
- Hintergrund
- Cytotoxic T lymphocytes (CTL's) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.
- Molekulargewicht
- 90-100kDa
- Gen-ID
- 6490
- UniProt
- P40967
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