SOX10 Antikörper (AA 115-269)

Produktnummer ABIN6940623

Produktdetails anti-SOX10 Antikörper

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Bindungsspezifität: AA 115-269

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOX10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Spezifität: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of ~55 kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietary)

Klon: SOX10-991

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HepG2 cells. Melanomas, breast carcinomas, gliomas.

Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Andere Bezeichnung: SOX10 (SOX10 Produkte)

Synonyme: DOM antikoerper, PCWH antikoerper, WS2E antikoerper, WS4 antikoerper, WS4C antikoerper, SOX-10 antikoerper, SOX10 antikoerper, dom antikoerper, ws4 antikoerper, Dom antikoerper, Sox21 antikoerper, SOX9 antikoerper, cls/sox10 antikoerper, zgc:100757 antikoerper, SRY-box 10 antikoerper, SRY box 10 antikoerper, SRY-box 10 L homeolog antikoerper, SRY (sex determining region Y)-box 10 antikoerper, SOX10 antikoerper, Sox10 antikoerper, sox10.L antikoerper, sox10 antikoerper

Molekulargewicht: 49-58kDa

Gen-ID: 6663

UniProt: P56693

Pathways: Chromatin Binding