BRCA1 Antikörper (AA 445-620)

Produktnummer ABIN6940661

Produktdetails anti-BRCA1 Antikörper

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Bindungsspezifität: AA 445-620

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BRCA1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human BRCA1 protein fragment (around aa445-620) (exact sequence is proprietary)

Klon: BRCA1-1398

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: A431 or HeLa cells. Breast, Ovarian or Renal Cell Carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (2-5 μg/mL for 60 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Andere Bezeichnung: BRCA1 (BRCA1 Produkte)

Hintergrund: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

Molekulargewicht: 220kDa

Gen-ID: 672

UniProt: P38398

Pathways: Zellzyklus, DNA Reparatur, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus